The Loizou lab is a DNA repair research group. Our vision is to piece together the intricate puzzle that encompasses the human DNA damage response at the cellular level, hence providing a complete understanding of how such pathways go wrong in disease states, with a strong emphasis on cancer.
To achieve this, the team uses genome-scale approaches, based around genetics, genomics, proteomics and chemical biology. We hypothesize, and have proven, that by investigating the regulatory pathways of DNA repair taking unbiased approaches, we can:
1. Advance our knowledge of how cells respond to DNA damage,
2.Uncover genetic interactions required for DNA repair and cellular survival and
3. Unravel the impact on the genome upon engagement of DNA damage and DNA repair pathways. Hence, we envisage that our research will shed light on the mechanisms leading to cellular transformation and pave the way for better treatments for cancer.
Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair
Ferreira da Silva J, Oliveira GP, Arasa-Verge EA, Kagiou C, Moretton A, Timelthaler G, Jiricny J, Loizou JI.
Nat Commun. 2022 Feb 9;13(1):760.
Mapping the Human Kinome in Response to DNA Damage
Owusu M, Bannauer P, Ferreira da Silva J, Mourikis TP, Jones A, Májek P, Caldera M, Wiedner M, Lardeau CH, Mueller AC, Menche J, Kubicek S, Ciccarelli FD, Loizou JI.
Cell Rep, 26, 555-563 e556, 2019
Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48
Velimezi G, Robinson-Garcia L, Muñoz-Martínez F, Wiegant WW, Ferreira da Silva J, Owusu M, Moder M, Wiedner M, Rosenthal SB, Fisch KM, Moffat J, Menche J, van Attikum H, Jackson SP, Loizou JI.
Nat Commun, 9, 2280, 2018
Validating the concept of mutational signatures with isogenic cell models
Zou X, Owusu M, Harris R, Jackson SP, Loizou JI*, Nik-Zainal S*.
Nat Commun, 9, 1744, 2018;
Repair of UV-Induced DNA Damage Independent of Nucleotide Excision Repair Is Masked by MUTYH
Mazouzi A, Battistini F, Moser SC, Ferreira da Silva, Wiedner M, Owusu M, Lardeau C-H, Ringler A, Weil B, Neesen J, Orozco M, Kubicek S, Loizou JI.
Mol Cell, 68, 797-807 e797, 2017
Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia
Moder M, Velimezi G, Owusu M, Mazouzi A, Wiedner M, Ferreira da Silva J, Robinson-Garcia L, Schischlik F, Slavkovsky R, Kralovics R, Schuster M, Bock C, Ideker T, Jackson SP, Menche J, Loizou JI.
Nat Commun, 8, 1238, 2017